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. 2011 Sep 27;286(45):39417–39430. doi: 10.1074/jbc.M111.295188

FIGURE 6.

FIGURE 6.

Effects of Pi and iron concentration on secretion and expression of proteins from T3SS and T6SS in E. tarda PPD130/91 Δfur mutant. The effects of Pi and iron concentrations on the amounts of ECPs (A) and TCPs (B) from T3SS (EseB, EseC, and EseD) and T6SS (EvpC and EvpP) were determined by growing E. tarda Δfur mutant in DMEM or the same medium containing 20 mm KH2PO4 and/or 10 μm FeSO4. The results were determined using Western blotting. C, the effects of Pi and iron concentration on the β-galactosidase activities of the reporter genes phoB-LacZ, phoU-LacZ, esrB-LacZ, esrC-LacZ, and fur-LacZ in E. tarda PPD130/91 Δfur mutant were determined by culturing the cells in DMEM (white bar) or the same medium containing 20 mm KH2PO4 (light gray bar), 10 μm FeSO4 (dark gray bar), or 20 mm KH2PO4 + 10 μm FeSO4 (black bar). Values represent mean ± S.E., n = 3, by one experiment.