TABLE 1.
Properties of recombinant chemerin isoforms and chemerin-derived peptides
The C-terminal sequences of all chemerin isoforms and the equivalent peptides tested in this study were displayed, as well as the EC50 values on human CMKLR1/L1.2 cells determined by Ca2+ mobilization and chemotaxis assay. Data are shown as mean ± S.D. and calculated from at least four independent experiments.
| C-terminal Sequence | Amino acids | Molecular mass | pI | Ca2+ flux | Chemotaxis | |
|---|---|---|---|---|---|---|
| Da | nm | nm | ||||
| Chem163S | –HSFYFPGQFAFSKALPRS | 143 | 16,529.9 | 8.89 | 54.2 ± 19.9 | 68.4 ± 46.2 |
| Chem158K | –HSFYFPGQFAFSK | 138 | 16,005.3 | 8.81 | 65.2 ± 13.2 | 45.3 ± 25.0 |
| Chem157S | –HSFYFPGQFAFS | 137 | 15,877.1 | 8.60 | 1.17 ± 0.74 | 3.15 ± 1.57 |
| Chem156F | –HSFYFPGQFAF | 136 | 15,790.0 | 8.60 | NDa | ND |
| Chem155A | –HSFYFPGQFA | 135 | 15,642.8 | 8.60 | >1,000 | 381 ± 253 |
| 15-mer | YFPGQFAFSKALPRS | 15 | 1,715.9 | 9.99 | >1,000 | >1,000 |
| 10-mer | YFPGQFAFSK | 10 | 1,191.3 | 8.59 | >1,000 | 849 ± 740 |
| 9-mer | YFPGQFAFS | 9 | 1,063.1 | 5.52 | 5.92 ± 3.26 | 6.38 ± 3.03 |
| 11-mer | HSFYFPGQFAF | 11 | 1,347.5 | 6.74 | 343 ± 107 | 288 ± 200 |
| FA | HSFYFPGQFA | 10 | 1,200.3 | 6.74 | >1,000 | >1,000 |
a ND, not determined.