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. 2011 Sep 12;286(45):39663–39672. doi: 10.1074/jbc.M111.273581

FIGURE 5.

FIGURE 5.

Effect of TRPV1 antagonists on heat-activated TRPV1 chimeric and wild-type isoforms using manual patch-clamp electrophysiology. A–D, representative plots of heat-activated current measured at +100 mV during heat ramp in HEK293 cells transfected with hTRPV1WT, rTRPV1WT, h-rTMD5/6-h, r-hTMD5/6-r in the absence and presence of JYL-1421 (0.33 μm). Heat ramp is depicted under each current plot (heat ramp from 23 °C to 47 °C). The compound was pre-applied for 2 min before heat activation. E, summary of effect of JYL-1421 on heat activated hTRPV1WT, rTRPV1WT, h-rTMD5/6-h, r-hTMD5/6-r channels. Each bar is the mean ± S.E. (n = 5 for hTRPV1WT, n = 7 for rTRPV1WT, n = 6 for h-rTMD5/6-h, and n = 7 for r-hTMD5/6-r).