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. 2011 Sep 8;286(50):43394–43404. doi: 10.1074/jbc.M111.235127

FIGURE 8.

FIGURE 8.

siRNA-mediated knockdown of desmin induces hypertrophy through the Egr-1/miR-26a/GSK-3β pathway in human ASMCs. Normal HASMCs and reHASMCs were transfected with anti-miR-26a or NS-miR. A–E, 48 h after transfection, cell size (A), protein/DNA ratio (B), smooth muscle-specific contractile proteins (C), proliferation (D), and GSK-3β protein levels (E) were determined. F, normal HASMCs and reHASMCs were transfected with Egr-1 siRNA or Egr-1 nonspecific siRNA (Egr-1NS-siRNA). miR-26a levels were determined by qPCR. Gel pictures are representative of three separate experiments. Each bar indicates mean ± S.E. (n = 3). *, p < 0.05.