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. 2011 Sep 8;286(50):43394–43404. doi: 10.1074/jbc.M111.235127

TABLE 1.

Primers used in PCR

Primer name Sequence 5′–3′ Underline Purpose
miR-26a-C-F GGATCCGTGATATCACAAGGTCCCAG BamHI Cloning
miR-26a-C-R AAGCTTCTACATGCAAAGGGCAGGAG HindIII Cloning
GSK-3β-F1 CACCCATTTATCATTAACCTAGCA Cloning
GSK-3β-R1 TCTTTCCAAACGTGACCAGT Cloning
GSK-3β3′-UTR-F GCTAGCCCCGTGACATTGTTAGCAGTT SacI Cloning
GSK-3β3′-UTR-R CTCGAGTGCCAGTGTCTTTACGTCCA XhoI Cloning
miR-26a 5′-UTR-F1 GGTACCCATAGACTGGGTGGCGAGTT KpnI Cloning
miR-26a 5′-UTR-R1 GAGCTCATCGACGTAAGGCCTCTTGA SacI Cloning
miR-26a 5′-UTR-F2 GGTACCCCTCTGGTGAAGAGCTACTGAA KpnI Cloning
miR-26a 5′-UTR-R2 GAGCTCAGCCTGCACAAGTGTTCCTT SacI Cloning
miR-26a 5′-UTR-F3 GGTACCGCTGGGATTTGAACTCAGGA KpnI Cloning
miR-26a 5′-UTR-R3 GAGCTCTGACAGATGCATATGGAGCAC SacI Cloning
ChIP-F CCCCCTGCTGTCTAGTTGAG qPCR
ChIP-R CAGCCTCCACAGTAGCCTTC qPCR
Ctdsp2-F GCCAAGTATGCTGACCCTGT qPCR
Ctdsp2-R GTTTTCCTCAGGTCCCTTCC qPCR
GSK-3β-F TTCGCCGTCCTTAACTCTTG qPCR
GSK-3β-R CGAAGGTAGCCGAACAGAAG qPCR
GAPDH-F TGTTCCTACCCCCAATGTGT qPCR
GAPDH-R CCTGCTTCACCACCTTCTTG qPCR