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. 2011 Oct 23;286(50):43495–43505. doi: 10.1074/jbc.M111.286104

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Pex18p is ubiquitinated at a conserved cysteine residue. Pex14p-TEV-ProtA was isolated from oleic acid-induced wild-type cells in the absence or presence of NEM (A), upon expressing either plasmid-encoded Ub or mycUb in the presence of 20 mm NEM (B), or from pex18Δ21Δ expressing either wild-type Pex18p or mutated Pex18p as indicated as well as pex18Δ21Δ4Δ expressing wild-type Pex18p (C). Equal portions of the obtained eluate fractions were analyzed by immunoblotting for the presence of Pex18p and its modified species. *, cross-reactive band.