Both RelA and TAp63α mediated cell death pathways. A and B, MCF10A cells transfected with the pCMV4-RelA expression cassette (0–1.5 μg) for 24 h. A, immunoblotting with the indicated antibodies. B, MTT assay. C and D, MCF10A cells transfected with the RelA shRNA (0–1.5 μg) for 48 h followed by immunoblotting with the indicated antibodies. C, MCF10A cells were transfected with the 1 μg RelA or p65Res constructs for 24 h followed by transfection with RelA shRNA (0–1.5 μg) for 24 h and immunoblotted with the indicated antibodies. D, MTT assay. *, p ≤ 0.05 (n = 3) compared with control (untransfected cells or scrambled shRNA). Error bars, S.D.