TABLE 2.
Structural parameters from small-angle X-ray scattering data
| Molecule | Lhx4-Isl2 | Lhx3-Isl1 | Lhx3-Isl2 |
|---|---|---|---|
| Dmax (Å) | 77 | 89 | 75 |
| [protein] (mg ml−1) | 3.10 ± 0.08a | 5.00 ± 0.11a | 2.57 ± 0.07a |
| I(0) (cm−1) | 0.0517 (± 0.0003)b | 0.0698 (± 0.0003)b | 0.0400 (± 0.0003)b |
| υ (cm3 g−1) | 0.719 | 0.718 | 0.720 |
| ΔρM (× 1010 cm−2) | 2.23 | 2.24 | 2.22 |
| Rg (Å) | 23.5 (± 0.3)b | 26.9 (± 0.2)b | 22.6 (± 0.4)b |
| MMexpc (kDa) | 20.2 ± 0.5 | 16.8 ± 0.4 | 19.0 ± 0.6 |
| MMpr (kDa) | 18.9 | 19.0 | 18.8 |
| MMexp/MMpr | 1.07 | 0.88 | 1.01 |
a Error is 1 S.D.
b I(0) and Rg were derived from P(r) using GIFT. The errors for these values were obtained by analyzing the Guinier region of the scattering in PRIMUS. GIFT does not produce errors for these values.
c MMexp = I(0)NA/[protein]ΔρM2.