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. 2011 Oct 11;286(50):42959–42970. doi: 10.1074/jbc.M111.295758

FIGURE 1.

FIGURE 1.

The inhibitory profile of tocilizumab is independent of IL-6R expression. A, equal numbers of Ba/F3-gp130 cells were cultured for 2 days with 10 ng/ml Hyper-IL-6, 10 ng/ml human, or 10 ng/ml murine IL-6. As a control, Ba/F3-gp130 cells were cultured without any cytokine. B, equal numbers of Ba/F3-gp130, Ba/F3-gp130-hIL-6R, or Ba/F3-gp130-mIL-6R cells were cultured for 2 days with 10 ng/ml human IL-6 and increasing concentrations of the anti-human-IL-6R antibody tocilizumab (0.001–10,000 μg/ml). C, equal numbers of Ba/F3-gp130 or Ba/F3-gp130-hIL-6R cells were cultured for 2 days with 10 ng/ml hIL-6 plus increasing concentrations of shIL-6R as indicated. D, equal numbers of Ba/F3-gp130, Ba/F3-gp130-hIL-6R, or Ba/F3-gp130-mIL-6R cells were cultured for 2 days with 10 ng/ml human IL-6 plus 200 ng/ml shIL-6R and increasing concentrations of the anti-human-IL-6R antibody tocilizumab (0.001–10,000 μg/ml). Statistical analysis was carried out using a paired Student's t test, and statistical significance was p < 0.01 (**). The proliferation measured with the lowest inhibitor concentration was about the same as without inhibitor.