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. 2011 Oct 11;286(50):42959–42970. doi: 10.1074/jbc.M111.295758

FIGURE 3.

FIGURE 3.

sgp130Fc specifically inhibits IL-6 trans-signaling on cells lacking membrane-bound IL-6R. A, equal numbers of Ba/F3-gp130 cells were cultured for 2 days with 10 ng/ml Hyper-IL-6, 10 ng/ml hIL-6 plus 200 ng/ml shIL-6R, or 10 ng/ml mIL-6 plus 200 ng/ml smIL-6R. Inhibition of growth was achieved by increasing concentrations of sgp130Fc (0.001–10,000 ng/ml). B, equal numbers of Ba/F3-gp130 cells were incubated with the indicated cytokines for 30 min after 6 h of serum starvation. Western blot analysis of cell lysates was performed with an antibody against phospho-STAT3 and against STAT3 as loading control. C and D, equal numbers of Ba/F3-gp130-hIL-6R or Ba/F3-gp130-mIL-6R cells were cultured for 2 days with either 10 ng/ml human or murine IL-6 plus increasing concentrations of sgp130Fc (0.001–10,000 ng/ml). Statistical analysis was carried out using a paired Student's t test, and statistical significance was p < 0.05 (*) or p < 0.01 (**). The proliferation measured with the lowest inhibitor concentration was about the same as without inhibitor.