FIGURE 7.
Effects of mutations on ATP and Ca2+ uptake. The VNUT mutants were purified, and ATP uptake (left panel) and Ca2+ uptake (right panel) were measured at 2 min after the addition of valinomycin (Val). Coomassie Brilliant Blue staining of the purified wild-type and mutant VNUT-containing samples is shown in the inset. Error bars represent means ± S.D. (n = 3–5).