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. 2011 Oct 25;286(50):43527–43536. doi: 10.1074/jbc.M111.273573

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Sedimentation velocity analysis. The continuous distribution of sedimentation coefficients c(s) for 14-3-3ζ alone (green), RGS3 alone (red), 14-3-3ζ, and pRGS3 mixed in the molar ratio 2:1 (black), 14-3-3ζ and pRGS3 mixed in the molar ratio 2:2 (orange), and 14-3-3ζ and unphosphorylated RGS3 mixed in the molar ratio 2:2 (blue) is shown. The weight-averaged sedimentation coefficient (s_w) of 5.0 s for the 14-3-3ζ·pRGS3 complex corresponds to the molecular mass of ∼84 kDa, indicating a 2:1 stoichiometry (dimer of 14-3-3ζ binds one molecule of pRGS3). Values of s_w were obtained by integration of c(s) curve.