TABLE 2.

Mean excited state lifetimes of AEDANS-labeled single-Cys mutants of pRGS3

Single-Cys RGS3 mutant	Mean excited state lifetime τmeana
	pRGS3	pRGS3 + 14-3-3ζ
	ns
AEDANS Cys-276	13.7	12.7
AEDANS Cys-285	14.2	13.6
AEDANS Cys-427	14.3	13.8
AEDANS Cys-456	9.8	12.7
AEDANS Cys-480	12.4	12.2

^a The S.D. value is ±0.1 ns.