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Effect of shift reagents on glycine 1H-15N HSQC signals of Hsp12 in presence of 200 mm SDS. A, water-soluble shift reagent, 10 mm Gd(DTPA-BMA). B, no shift reagent (control). C, lipid-soluble shift reagent, 10 mm 16DSA. Spectra were recorded at 40 °C on a 900 MHz spectrometer equipped with a cryogenic probe.
