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. 2011 Oct 17;286(50):42900–42910. doi: 10.1074/jbc.M111.274456

FIGURE 2.

FIGURE 2.

Capacity of the various mutated virus strains to bind to sCD4 as percentage of wild-type virus and to induce multinuclear syncytia in co-cultures. A, the N260Q mutant virus strain has a significant decrease of binding (*, p < 0.05) compared with wild-type virus. The decreased binding of the mutant N239Q and N354Q gp120 virus to sCD4 was not significant versus wild-type virus under both treatments. Statistical analysis was performed with the two-tailed Student's t test. Data are means ± S.D. (error bars). B, giant cell formation between HIV-1 (WT or mutant)-transfected 293T cells and uninfected U87.CD4.CCR5.CXCR4 cells. Left, co-culture of 293T cells, expressing wild-type envelope glycoprotein with U87.CD4.CCR5.CXCR4 cells. Middle, co-culture of 293T cells expressing the envelope glycoprotein containing the N260Q mutation with U87.CD4.CCR5.CXCR4 cells. Right, control condition. 293T cells transfected with HIV-1 NL4.3ΔEnv_EGFP (lacking env) and co-cultured under the same conditions as in A and B with U87.CD4.CCR5.CXCR4 cells.