TABLE 1.
Primers for real-time qPCR
| Region | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Product length | Annealing temperature |
|---|---|---|---|---|
| bp | ºC | |||
| IL-17A | ACCAATCCCAAAAGGTCCTC | CACTTTGCCTCCCAGATCAC | 110 | 60 |
| 18 S rRNA | ACTCAACACGGGAAACCTCA | AACCAGACAAATCGCTCCAC | 110 | 60 |
| IL17A_CNS1 | CTGCCCTTCCCATTTTCCTT | GTTCAGGGGTGACACCATTT | 456 | 55 |
| IL17A_PP | ACATGATATTGACCCATAGC | AGGGCAGAAATTCATGTTCC | 456 | 55 |
| IL17A_PP-CRE | TCATTGGGGGCGGAAATTTTAACCA | GGGCTTTTCTCCTTCTGTGGTCAC | 90 | 55 |
| IL17A_CNS2 | GCGATGCTCTTGCTGATTTG | CCTGGATCTCCATAGTCAGAAC | 352 | 55 |