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. 2011 Oct 22;286(50):42818–42829. doi: 10.1074/jbc.M111.253203

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — MTF-1 cross-interacts with each other through SIM and the interaction can be disrupted by zinc treatment. HA-tagged MTF-1 and MTF-1-His₆, SIM-3A-His₆ (A) or K627R-His₆ (B) were co-expressed in CHO K1 cells. Following immunoprecipitation (IP) with anti-His₆ antibodies, the presence of HA-tagged MTF-1 was visualized with anti-HA antibodies on Western blot (IB). HA-tagged MTF-1 and His₆-tagged MTF-1 were co-expressed in the CHO K1 cells and treated with various concentrations of zinc for 6 h (C) or 100 μm zinc for various time intervals (D). The degree of MTF-1 cross-interaction was analyzed with the anti-His₆ antibody after cell extracts were immunoprecipitated with HA antibodies.