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. 2011 Oct 22;286(50):42818–42829. doi: 10.1074/jbc.M111.253203

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Demonstration of SIM-dependent MTF-1 complex formation with gel permeation chromatography. His₆-tagged MTF-1, K627R, or SIM-3A were expressed in CHO K1 cells with or without 100 μm zinc treatment for 6 h. Cell extracts were prepared and separated on an analytical Superose 6 HR 10/30 column. Equal amounts of eluent from each fraction were concentrated and analyzed by immunoblotting using anti-His₆ antibodies. The elution positions of the molecular markers were indicated on top of the panel. MTF-1 can be detected after fraction number 26.