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. 2011 Oct 22;286(50):42818–42829. doi: 10.1074/jbc.M111.253203

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Localization of MTF-1 complexes. A, Gal4-TAD or NLSm-TAD were expressed in CHO K1 cells. Cytosolic and nuclear extracts were prepared and cellular distribution of the expressed proteins was analyzed by immunoblotting (IB). Tubulin and lamin were employed as cytosolic (Cy) and nuclear markers (Nu), respectively. B, Gal4-TAD or NLSm-TAD plasmids harboring different tags were co-transfected into CHO K1 cells and cell extracts were prepared. Cross-interaction of Gal4-TAD or NLSm-TAD was analyzed in cells treated with or without 100 μm zinc for 6 h.