FIGURE 3.

Subcellular localization of NLRP7 and co-localizations. A–C, expression of NLRP7 and IL-1β in a monocyte after LPS stimulation. DNA was counterstained with DAPI (blue). D, NLRP7 is distributed homogeneously in the cytoplasm of unstimulated THP1 cells, whereas it has a predominantly polar signal in stimulated PBMCs (E–G). In E, note the faint microtubule-like network staining emerging from the NLRP7 polar signal. Stimulated PBMCs show overlap between the localization of NLRP7 protein and protein disulfide isomerase (PDI), a marker of the reticulum endoplasmic (H–J). Co-localization of NLRP7 protein in LPS-stimulated THP1 cells with giantin, a marker of the cis-Golgi (K–M). Co-localization in PBMCs of NLRP7 protein with γ-tubulin, a marker of the MTOC (N–P).