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. Author manuscript; available in PMC: 2012 Nov 29.
Published in final edited form as: Biochemistry. 2011 Nov 2;50(47):10203–10214. doi: 10.1021/bi200964p

Figure 5.

Figure 5

Reconstitution of the CF IA complex. A. Size exclusion chromatography of Pcf11/Clp1, Rna14/Rna15, and the assembled CF IA. B. SDS PAGE of the individual sub-complexes and the peak fractions of the wt CF IA complex. C. Addition of recombinant CF IA to TAP-purified CPF enhances polyadenylation activity. D. Addition of recombinant CF IA to a yeast extract with the Rna14-ES mutation rescues both cleavage and polyadenylation activity. E. Recombinant CF IA rescues activity when combined with yeast extract from the Pcf11-2 strain.