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Limited proteolysis. A. Trypsin cleavage of Rna14/Rna15. Lanes: M, molecular weight markers; U, undigested Rna14/Rna15; 1, Trypsin digest after 1hr; 2, after 2 hours; 3, after 3 hours; ft; flow through of nickel affinity column; pk, peak fraction from the nickel affinity column. The digested Rna14 product has an apparent molecular weight of ~70 kDa. B. Chymotrypsin cleavage of Rna14/Rna15.
