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Complementation of A. nidulans bioF deletion mutant with GFP-bioF chimeric constructs. A. nidulans biotin-prototrophic strain FGSC A1145 (dish 1), the ΔbioF mutant (dish 2), and the ΔbioF mutant transformed with either the GFP-BioF-FL (dish 3) or GFP-BioF-TR (dish 4) construct were grown on minimal medium deficient in biotin and supplemented with avidin (upper) or supplemented with biotin (lower).
