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Complementation of E. coli biotin-auxotrophic mutant bioF103 with GFP-bioF constructs. The E. coli mutant bioF103 strain was transformed with a full-length A. nidulans bioF cDNA cloned in vector pTRC99A (sector 1), empty vector pTRC99A (sector 2), and either the GFP-BioF-FL (sector 3) or GFP-BioF-TR (sector 4) chimeric construct, both in pTRC99A. The medium in the left dish was biotin-deficient and contained avidin, whereas that in the right dish was supplemented with biotin.
