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β-Oxidation cycle is not required for biotin synthesis in E. coli. Cells were grown on medium supplemented with biotin (right) or on biotin-depleted medium containing avidin (left). The strains used were biotin-auxotrophic ΔbioD (sector 1); control BW25113 (sector 2); and deletion mutants ΔfadA (sector 3), ΔfadB (sector 4), ΔfadE (sector 5), ΔfadK (sector 6), ΔfadJ (sector 7), and ΔfadI (sector 8). Plates were incubated under aerobic (upper) or anaerobic (lower) conditions.
