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. 2011 Oct 13;286(49):42150–42161. doi: 10.1074/jbc.M111.308049

FIGURE 9.

FIGURE 9.

Induction of heat shock response by 6-HITC. A, RT-PCR analysis of Hsp70 in Caco-2 cells treated with 6-HITC. B, immunoblot analysis of intracellular and extracellular Hsp70 in Caco-2 cells treated with 6-HITC. Hsp70 was analyzed by immunoblot analysis using the goat anti-Hsp70 polyclonal antibody. C, effects of specific siRNA of HSF-1 upon induction of Hsp70 by 6-HITC. The cells were treated for 48 h with the indicated siRNA as described above prior to incubation with 6-HITC for 8 h. In A and B, the cells were treated with 25 μm 6-HITC for the indicated time intervals.