FIGURE 3.

PrxIV forms stable decamer in both reduced and oxidized conditions. A, schematic representation of the dynamic changes between oligomeric states of peroxiredoxins in different redox conditions and their possible functions. The roman numbers represent different peroxiredoxins. B, size exclusion chromatograms of PrxIII and PrxIV carried out under normal or reducing conditions on a Superdex 200 column. The column was pre-equilibrated with buffer with or without DTT as indicated. C, detailed representation of the dimer-dimer interface in PrxIV. The hydrophobic side chains are highlighted. Blue and red represent different chains of PrxIV.