TABLE 2.

¹H NMR data of acetylated and non-acetylated methyl-β-d-xylopyranoside

Data are as viewed after partial deacetylation of methyl 2,3,4-tri-O-acetyl-β-d-xylopyranoside by Axe2 in citrate phosphate buffer (pH 6.8) at room temperature. The chemical shifts of the fully acetylated and deacetylated forms of the sugar are also given in D₂O, calibrated with DSS. The chemical shifts were determined by one-dimensional selective TOCSY using a Bruker Avance AV-III 600 spectrometer (one-dimensional homonuclear Hartman-Hahn transfer using the MLEV-17 sequence for mixing). Data were collected with 32 shots, a 83.2-μs dwell time, 65,536 real points, a 4-s relaxation time, and 20–80-ms mixing times.

	H1	H2	H3	H4	H5a	H5b
Methyl 2,3,4-tri-O-acetyl-β-d-xylopyranoside	4.64	4.83	5.16	4.95	4.12	3.56
Methyl 3,4-di-O-acetyl-β-d-xylopyranoside	4.42	3.51	5.03	4.89	4.04	3.47
Methyl 2-mono-O-acetyl-β-d-xylopyranoside	4.46	4.61	3.58	3.65	3.95	3.30
Deacetylmethyl-β-d-xylopyranoside	4.26	3.18	3.37	3.55	3.90	3.26
Methyl 2,3,4-tri-O-acetyl-β-d-xylopyranosidea	4.7	4.88	5.22	5.01	4.16	3.60
Methyl-β-d-xylopyranosidea	4.31	3.23	3.42	3.60	3.96	3.31

^a In D₂O calibrated with DSS (δ_DSS = 0 ppm).