TABLE 3.
1H NMR data of acetylated and non-acetylated methyl-β-d-glucopyranoside
The chemical shifts were determined in D2O calibrated with DSS (δDSS = 0 ppm) using one-dimensional selective TOCSY with a Bruker Avance AV-III 600 spectrometer (one-dimensional homonuclear Hartman-Hahn transfer using the MLEV-17 sequence for mixing). Data were collected with 32 shots, a 83.2-μs dwell time, 65,536 real points, a 4-s relaxation time, and 20–80-ms mixing times.
| H1 | H2 | H3 | H4 | H5 | H6a | H6b | |
|---|---|---|---|---|---|---|---|
| Methyl 2,3,4,6-tetra-O-acetyl-β-d-glucopyranoside | 4.75 | 4.95 | 5.35 | 5.12 | 4.05 | 4.40 | 4.23 |
| Methyl-β-d-glucopyranoside | 4.37 | 3.25 | 3.48 | 3.36 | 3.44 | 3.92 | 3.71 |