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DNA methylation in the proximal promoter of CHRNA7. DNA isolated from SH-SY5Y, SK-N-BE, HeLa, and SH-EP1 cells was used for sodium bisulfite conversion to identify methylated CpG islands within the CHRNA7 promoter region. The proximal promoter region is indicated by the location of the CpG islands and the percentage of samples showing methylation. SH-EP1 cells show heavy methylation over the region where the original AP-2α binding site is located (−71).
