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Monoclonal antibodies to wheat eIF4F. Mouse monoclonal antibodies were raised to wheat native eIF4F. The ability of individual monoclonal antibodies to react with native (n) eIF4F and recombinant (r) eIF4F was tested by Western blotting. Mouse ascites fluid (1/1000) was incubated overnight at 4 °C, and the second antibody was goat anti-mouse HRP (1/20,000, Kirkegaard-Perry). The chemiluminescent substrate was Super Signal West Pico (Thermo-Pierce).
