FIGURE 4.

Comparison of recombinant and native eIF4F and eIFiso4F in translation. Each 100-μl translation reaction contained 5 pmol of capped rabbit β-hemoglobin mRNA and the indicated amounts of eIF4F or eIFiso4F complexes (■, solid line, native eIF4F; □, dashed line, recombinant eIF4F; ●, solid line, native eIFiso4F; △, dashed line, recombinant eIFiso4F) as described under “Experimental Procedures.” The amount of [¹⁴C]leucine incorporated in the absence of cap-binding complexes was 3.8 pmol. Each point represents the average of three experiments, and the error bars are indicated.