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. 2011 Sep 30;286(49):42566–42574. doi: 10.1074/jbc.M111.280099

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Displacement assays. To determine whether the correct cap-binding protein is able to displace a mismatched cap-binding protein in a mixed complex, the indicated amounts of eIF4E or eIFiso4E were added to 2.5 pmol of mixed complexes, eIF4F, or eIFiso4F. A and B were probed with mouse monoclonal antibody (8F7) to eIF4E (1/1000). The blots were stripped and probed with rabbit polyclonal antibody raised to native gel-purified eIFiso4E (1/1000) shown in C and D. The antibody to native eIFiso4E displays a small amount of cross-reactivity with eIFiso4G (see D, eIFiso4G-eIF4E with no eIFiso4E added).