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. 2011 Oct 24;286(49):42470–42482. doi: 10.1074/jbc.M111.309252

FIGURE 7.

FIGURE 7.

The role of ATM kinase activity, DNA-PKcs kinase activity, and RAD50 during HDR is unaffected by DSB transcription context. Cells with the g4DRGFP reporter were transfected with an I-SceI expression vector under TxON or TxOFF conditions, and treated with ATMi, PKi, DMSO, or were depleted of RAD50 via siRNA. HDR values are shown relative to parallel control samples (e.g. DMSO-treated or siCTRL-treated) from the same Tx condition. For both TxON and TxOFF conditions, shown is the frequency of HDR of ATMi-treated HEK293-A7 (5 μm) and U2OS-R1 cells (10 μm), PKi-treated U2OS-R1 cells (20 μm), and RAD50-depleted U2OS-R1 cells. Asterisks indicate a statistically significant decrease in HDR compared with parallel control samples in the same Tx condition (p < 0.0018, except siRAD50 TxOFF, p = 0.03).