FIGURE 7.

Correlation of H3K9me3 and H3K27me3 at CDYL target promoters. A, correlation of H3K27me3 and H3K9me3 within CDYL binding sites (right panel) and randomly selected genomic regions (left panel). Numbers in the upper right quadrant indicate total sites with both H3K27me3 and H3K9me3 present (the cut-off value is 2). B and C, ChIP-coupled real-time RT PCR assays were performed with the indicated antibodies in MCF-7 cells. Among the regions examined, the VGF promoter apparently enriched more H3K9me3 modifications and the MYT1 promoter enriched more H3K27me3 modifications, while significant amounts of CDYL were present at both regions. Both NEUROD1 and HOXB3 promoters showed coexistence of H3K9me3 and H3K27me3 modifications, with mild enrichment of CDYL at the NEUROD1 promoter and undetectable CDYL at the HOXB3 promoter. The transcriptional active PS2 promoter was used as a control in these experiments.