Lipid accumulation in heart from Cbs+/− mice with diet-induced obesity. Triglyceride (A), cholesteryl ester (B), total phospholipids (C), and free cholesterol (D) concentrations in heart from Cbs+/− and Cbs+/− mice fed chow or the HFD. Data is presented as means ± S.E., n = 5–6 mice per diet/genotype group. Open bars, Cbs+/+ mice; filled bars, Cbs+/− mice. Significance was determined by two-way ANOVA.