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. 2011 Oct 23;286(49):42483–42493. doi: 10.1074/jbc.M111.304592

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Mitochondrial dysfunction in heart from Cbs^+/− mice with diet-induced obesity. Mitochondrial dysfunction was assessed by quantifying the mitochondrion-encoded cytochrome b gene (mt-Cytb) copy number relative to the nucleus-encoded β-actin gene (Actb) copy number (A) and by assessing expression of Pgc-1α (B). C, expression of Acox-1. Data are presented as means ± S.E., n = 5–6 mice per diet/genotype group. Open bars, Cbs^+/+ mice; filled bars, Cbs^+/− mice. Inset depicts representative immunoblots for analyzed proteins in comparison with β-actin bands from the same blot. Significance was determined by two-way ANOVA.