FIGURE 8.

Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α₂AP (■) and 100 nm Plg/tcuPA with 15 nm α₂AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r² >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r² >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of oligo(dT)₆₅ incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)₆₅ per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r² >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)₆₅ added). D, effects of α₂AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)₆₅ (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)₆₅ (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r² >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)₆₅ added).