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. 2011 Oct 22;286(49):42248–42256. doi: 10.1074/jbc.M111.297630

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — MUC1 and p65 colocalize on cytokine gene promoters. A, nuclear DNA-bound proteins were isolated as described under “Experimental Procedures” and immunoblotted with anti-MUC1 antibodies Ab5 and 3C6. Nucleolin (C23) was included as a loading control. B, ChIP assay. Soluble chromatin was immunoprecipitated with anti-MUC1.CT antibody Ab5 or control IgG (NegC) and analyzed for κB consensus sites of promoters for the indicated cytokines. C, re-ChIP assay. Soluble chromatin was first immunoprecipitated with anti-MUC1.CT antibody Ab5 and then re-immunoprecipitated with anti-p65 antibody. D, in vivo ChIP assay. Soluble chromatin from tumor tissue was immunoprecipitated with anti-MUC1.CT Ab5 or control IgG and analyzed for κB consensus sites on promoters of the indicated cytokines.