FIGURE 5.

Negative effect of p65 inhibitor on MUC1-mediated activation of cytokine expression. A, RMA and RMA-MUC1/22TR cells were activated with ConA, and RMA-MUC1/22TR cells were either treated (+BAY) or not with BAY 11-7085 as described under “Experimental Procedures.” Total cell proteins were blotted with the indicated antibodies. Actin was used as a loading control. B, real-time PCR. The graphs represent the increase in cytokine mRNAs relative to the increase in GAPDH mRNA in the same sample ± S.D. of three independent experiments. *, p < 0.05. C, concentrations of secreted interleukins were measured by ELISA. *, statistically significant difference (p < 0.05).