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MUC1 containing the VNTR region interacts with cytokine promoters in a p65-dependent manner in breast cancer cells. A, cells were activated with TNF-α with or without BAY 11-7085 or left untreated. Soluble chromatin was immunoprecipitated with anti-MUC1 antibody 3C6 and analyzed for the indicated κB consensus sites. B, MCF-10A and MDA-MB-231 cells were activated with TNF-α or left untreated, fixed, and stained with anti-MUC1 antibody 4H5 (red). Nuclei were stained with DAPI (blue).
