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Nonconserved regions of SmDLG (A–C) and SmVKR1 (D and E) served as templates to synthesize digoxigenin-labeled antisense transcripts used as probes for in situ hybridization. SmVKR1 signals were exclusively detected in the ovary (o), whereas SmDLG signals were detected in the ovary and the vitellarium (v) of the female as well as in the testes (t) of the male. p, parenchyma. For control, a digoxigenin-labeled sense probe was used (F–H). (Scale bar, 50 μm.)
