TABLE 3.

Upstream binding partners of SmTK6 identified by Y2H analyses

Following sequencing of the 19 clones obtained from library screening, BlastX analysis revealed that they represented four groups (A–D) with homology to mucin (group A), the schistosome Src kinase SmTK3 (group B), a cell polarity protein with homology to Discs-large (group C), and proteins with no significant homology (group D). Appropriate full-length cDNA sequences were identified in the S. mansoni genome data set (see Ref. 36) or in the NCBI database (www.ncbi.nlm.nih.gov). Accession and Smp numbers, sizes of the full-length (fl) sequences, the appropriate e-values, and the clone numbers are given.

Clone group	Homology (accession/Smp number)	Size (bp)	e-value	Clone no.
A	Mucin homolog (H. sapiens, Q02817); fl: XP_002578516, Smp_161910	9849	1e−48	1, 6, 9, 10, 18, 36, 38, 40, 43, 45, 54, 59, 63
B	SmTK3 (Src kinase) (S. mansoni, CAE51198); fl: XP_002576744, Smp_151300	1944	0.0	50, 56, 58
C	Cell polarity protein/Discs-large homolog (H. sapiens, NP_001136172); fl: XP_002579880, Smp_170290	3432	2e−72	41
D	No significant homology			17, 66