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. 2011 Oct 17;286(49):42381–42389. doi: 10.1074/jbc.M111.259689

FIGURE 1.

FIGURE 1.

Zcchc11 knockdown inhibits cell proliferation in lung epithelial lines. A, immunoblot of cell lysates 48 h after transfection with Zcchc11-targeting or control siRNA. B, cell counts of H1299 cells transfected with the indicated siRNAs. Error bars represent S.E. from three experiments. **, p < 0.01 by matched-pair two-way analysis of variance with the Bonferroni post hoc analysis. C, staining with annexin V and 7-aminoactinomycin D (7-AAD) following treatment with control or Zcchc11-targeting siRNA or with camptothecin (positive control). Numbers indicate the percentage of cells in each section. D, genomic DNA content, as measured by PI staining, of H1299 cells following siRNA treatment. E, estimates of the percentage of H1299 cells in each phase of the cell cycle measured by PI staining. Error bars represent S.E. from six experiments. **, p < 0.01; ***, p < 0.001.