FIGURE 5.

Rb-independent regulation of passage through the G₁ checkpoint by Zcchc11. A, genomic DNA content of H2009 cells following treatment with control or Zcchc11-targeting siRNA and nocodazole to arrest proliferating cells in G₂. B, estimated percentage of H2009 cells in each stage of the cell cycle following siRNA and nocodazole treatment as in A. Error bars represent S.E. from three experiments. *, p < 0.05 by two-way analysis of variance with the Bonferroni post hoc test. C, immunoblots of late G₁ proteins from H1299, A549, and H2009 cells treated with control or Zcchc11-targeting siRNA. D, representative immunoblot of H1299 cells transfected with either EGFP or Zcchc11-GFP.