Bipolar stimulation-evoked amperometric current
Amperometric current was measured in each condition as labeled, and data were pooled. Cells were pulsed at Low Hz or High Hz stimulation. Under Low Hz stimulation, the splanchnic nerve was stimulated for 60 pulses at a frequency of 0.2 Hz. Under High Hz stimulation, the nerve was stimulated with four bursts of 15 pulses at a 2 Hz frequency with an interburst interval of 15 s. Stimuli were delivered at a constant voltage of 35 V for a 10-μs duration. Quantified pooled data were determined from baseline subtracted, integrated amperometric currents at the 60 s time point. The mean ± S.E. values are shown for each condition. Sample size is indicated in parentheses.