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. 2011 Nov 22;21(12):1662–1669. doi: 10.1038/cr.2011.182

Figure 5.

Figure 5

Delayed paternal mtDNA elimination by RNAi knockdown of genes important for the lysosomal functions in C. elegans. (A) smIs13; uaDf5/+ males were mated with N2 hermaphrodites on plates containing bacteria expressing dsRNA from the indicated genes. Multiple GFP-positive smIs13/+ 4-fold stage embryos were collected and subjected to PCR analysis to detect the presence of uaDf5 mtDNA. him-14 RNAi was used as an RNAi control to demonstrate that RNAi worked (producing higher frequency of males) but was not involved in delaying paternal mtDNA elimination. (B) A summary of the RNAi screen results. The genes undergoing RNAi treatment, their molecular identities, and the effects of RNAi treatments in retaining paternal uaDf5 mtDNA are shown.