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. 2011 Sep 23;21(1):1–9. doi: 10.1093/hmg/ddr417

Figure 6.

Figure 6.

Localization of WT-FUS in animals expressing mutant FUS. (A) Representative fluorescence microscopic images of TagRFP-WT-FUS and GFP-FUS P525L double transgenic animals with or without heat shock. The distribution of TagRFP-WT-FUS and GFP-FUS P525L proteins was not changed in double transgenic animals. After heat shock, GFP-FUS P525L protein formed numerous cytoplasmic granules (arrows), whereas TagRFP-WT-FUS remained in the nucleus. (B) Thrashing assay of the single and double transgenic animals. Co-expression of WT-FUS did not rescue motor activity impairment of double transgenic animals under basal conditions. After heat shock, WT-FUS animals recovered within 30 min, whereas both the single GFP-FUS P525L and double transgenic animals showed persistent motor impairment. NS, not significant; ***P< 0.001.