Figure 2.

shows schematic representation of the capsule genes of C serogroup in disrupted construction of NMB0065 gene with aaDA cassette. The NMB0065 gene was amplified using the 03-12-3 and 03-12-4 oligonucleotides (table 3) from C2135 strain. This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, USA), to generate the plasmid pLAN6. E. coli strain Z501 was transformed with plasmid pLAN6 resulting in the plasmid pLAN7. The ΩaaDA cassette was inserted into the BclI site of pLAN7 to generate plasmid pLAN45, which was transformed into the C2135 strain to generate the isogenic mutant strain M2 [18,19].