Figure 4. Recombinant protein expression by production cell lines derived by cassette exchange.

(A) Immunoblots of supernatants containing secreted recombinant glycoproteins. Molecular weights were calculated without signal peptides, glycosylations and GPI-anchors. The number of glycosylation sites is indicated (glyco.). Each lane represents a different subcell clone. Four subcell clones are shown for each protein with the exception of mLAMP2-dist, for which only one subcell clone was isolated. (B) Western blot of cell lysates of four NALP3 targeted subcell clones. Small amounts of recombinant human NALP3 were detected in all four soluble lysate fractions of the targeted cells, but not in the SWI3a-26 master cells (C). i = insoluble, s = soluble.